Thermo Fisher Scientific IL-7 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-7. Anti-Murine IL-7-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Murine IL-7 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-7 (500-P57) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-7.

Western Blot:
To detect Murine IL-7 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Murine IL-7 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P57BT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin 7 (IL-7) is a hematopoietic cytokine constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7 receptor to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.