Thermo Fisher Scientific IL-10 Polyclonal Antibody, PeproTech
마우스 IL-10을 인식하는 Rabbit Polyclonal Antibody로, Western blot, ELISA, IHC 등 다양한 면역분석에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. PeproTech 제품으로 높은 특이성과 재현성 제공.
- 카탈로그번호
- 500-P60-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-10 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 3 publications |
| Neutralization (Neu) | – | View 2 publications |
| In vitro Assay (IV) | – | View 1 publication |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
| Immunostaining (IS) | – | View 3 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-10 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930647 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-10. Anti-Murine IL-10-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-10 matrix.
Sandwich ELISA:
To detect Murine IL-10 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-10 (500-P60Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-10.
Western Blot:
To detect Murine IL-10 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-10 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages.
It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway.
Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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