Thermo Fisher Scientific M-CSF Polyclonal Antibody, PeproTech
Goat에서 유래한 Murine M-CSF 특이 폴리클로날 항체로, Western Blot과 Sandwich ELISA에 최적화됨. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 세포 성장 및 분화 연구에 적합하며 -20°C에서 보관.
- 카탈로그번호
- 500-P62G-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific M-CSF Polyclonal Antibody, PeproTech
Thermo Fisher Scientific M-CSF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Goat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine M-CSF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930659 |
Product Specific Information
AA Sequence of recombinant protein:
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine M-CSF. Anti-Murine M-CSF-specific antibody was purified by affinity chromatography employing an immobilized Murine M-CSF matrix.
Sandwich ELISA:
To detect Murine M-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine M-CSF (500-P62GBt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Murine M-CSF.
Western Blot:
To detect Murine M-CSF by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine M-CSF is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. It also induces VEGF secretion by macrophages, promoting endothelial progenitor cell mobilization and neovascularization.
M-CSF exists as several bioactive isoforms differing in potency and stability, synthesized as a membrane-spanning protein that can be surface-expressed or secreted. The protein forms a disulfide-bonded homodimer processed into glycoprotein or proteoglycan isoforms modified by chondroitin sulfate addition.
Binding to its receptor c-Fms (CSF-1R or CD115) triggers receptor dimerization, internalization, and degradation. Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte-macrophage populations. Human M-CSF shows activity on mouse cells, while mouse CSF shows no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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