
Thermo Fisher Scientific DARPP-32 Polyclonal Antibody
DARPP-32 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF 등 다양한 응용에 사용 가능. Mouse와 Rat 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse DARPP-32 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.11 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792923 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%).
- Positive Control: mouse brain, rat brain.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DARPP-32 is a dopamine (DA) and AMP-regulated ~32 kDa phosphoprotein associated with dopaminoceptive neurons.
The protein inhibits Protein Phosphatase I when phosphorylated on Thr34.
When phosphorylated on Thr75, DARPP-32 acts as an inhibitor of PKA.
Phosphorylation of DARPP-32 plays a critical role in the regulation of dopaminergic neurotransmission and is implicated in the actions of alcohol, caffeine, and Prozac®.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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