Thermo Fisher Scientific H3K14me2 Monoclonal Antibody (5HH3-2B10)
Histone H3K14me2 인식용 단일클론 항체로 Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합. Human 시료 반응성, Mouse IgG1 아이소타입. 합성 펩타이드 면역원 사용, 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
| Immunoprecipitation (IP) | 1:150 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5HH3-2B10 |
| Immunogen | Ovalbumin-conjugated synthetic peptide (TARKSTGG[dimethyl-K]APRC) corresponding to amino acids 6–17 of histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2633026 |
Product Specific Information
MA3-059 detects histone H3 in human samples and has been successfully used in western blot, immunoprecipitation, and immunofluorescence applications.
The immunogen is a synthetic peptide conjugated to ovalbumin corresponding to residues 6–17 of Human Histone H3, which is highly conserved across multiple species.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it binds DNA to form nucleosomes.
Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability.
Post-translational modifications of histones, such as methylation, acetylation, and phosphorylation, influence chromatin structure and gene expression.
The N-terminal tail of histone H3 can undergo multiple modifications that affect cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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