Thermo Fisher Scientific H4ac pan-acetyl (K5,K8,K12,K16) Monoclonal Antibody (3HH4-2C2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunocytochemistry (ICC/IF)

1:250

-

Immunoprecipitation (IP)

1:150

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3HH4-2C2

Immunogen

Ovalbumin conjugated synthetic peptide (MSGRG(acetyl-K)GG(acetyl-K)GLG(acetyl-K)GGA(acetyl-K)RHRKVLRC) corresponding to residues 1 to 24 of human histone H4 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H4ac pan-acetyl (K5,K8,K12,K16),uniProtId:P62805-1,ncbiNodeId:9606,antigenRange:1-24,antigenLength:103,antigenImageFileName:MA3-066_H4ac_pan-acetyl_K5,K8,K12,K16_P62805-1_House_mouse.svg,antigenImageFileNamePDP:MA3-066_H4ac_pan-acetyl_K5,K8,K12,K16_P62805-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2633028

Product Specific Information

MA3-066 detects acetylated histone H4 at Lysines 5, 8, 12 or 16 in human samples

MA3-066 has successfully been used in western blot, immunofluoresecence and immunoprecipitation applications.

Immunogen is a synthetic peptide conjugated to ovalbumin corresponding to residues 1 to 24 of human histone H4 which is conserved in a wide range of species.

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.