Thermo Fisher Scientific H4ac pan-acetyl (K5,K8,K12,K16) Monoclonal Antibody (3HH4-2C2)
Histone H4의 Lys5, Lys8, Lys12, Lys16 아세틸화 형태를 인식하는 단클론 항체. Western blot, ICC/IF, IP에 적합. Human 시료 반응성. Ovalbumin 결합 합성 펩타이드 면역원 사용. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:250 |
| Immunoprecipitation (IP) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3HH4-2C2 |
| Immunogen | Ovalbumin conjugated synthetic peptide (MSGRG(acetyl-K)GG(acetyl-K)GLG(acetyl-K)GGA(acetyl-K)RHRKVLRC) corresponding to residues 1–24 of human histone H4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.05% Sodium Azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet Ice |
| RRID | AB_2633028 |
Product Specific Information
- MA3-066 detects acetylated histone H4 at Lysines 5, 8, 12, or 16 in human samples.
- Validated for Western blot, immunofluorescence, and immunoprecipitation applications.
- Immunogen is a synthetic peptide conjugated to ovalbumin corresponding to residues 1–24 of human histone H4, conserved across species.
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and contributes to chromatin compaction.
This gene encodes a member of the histone H4 family, located in the large histone gene cluster on chromosome 6, and lacks introns and polyA tails, instead containing a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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