Thermo Fisher Scientific BrdU Monoclonal Antibody (BU-1)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:1,000-1:2,500

View 23 publications 23 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 16 publications 16 publications

Flow Cytometry (Flow)

1:100

View 5 publications 5 publications

Neutralization (Neu)

-

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Chicken, Mouse, Non-human primate

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

BU-1

Immunogen

5-iodouridine covalently coupled to ovalbumin.

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• DyLight 488
• Request custom conjugation

Form

Liquid

Concentration

0.05 mg/mL

Purification

Protein A

Storage buffer

0.01M HEPES with 0.15M NaCl, 30% glycerol

Contains

0.01% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10986341

Product Specific Information

MA3-071 detects 5-Bromo-2-deoxyuridine (BrdU) in many samples.

MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications.

The MA3-071 immunogen is 5-iodouridine covalently coupled to ovalbumin.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.