Thermo Fisher Scientific H3K9me2S10ph Monoclonal Antibody (6HH3-2C5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunoprecipitation (IP)

1:150

-

ChIP assay (ChIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6HH3-2C5

Immunogen

Ovalbumin conjugated synthetic peptide (AR[dimethyl-K][phospho-S]TGGAPRKQLC) correspeonding to aa 7-20 of histone H3 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3K9me2S10ph,uniProtId:P68431-1,ncbiNodeId:9606,antigenRange:7-20,antigenLength:136,antigenImageFileName:MA3-064_H3K9me2S10ph_P68431-1_House_mouse.svg,antigenImageFileNamePDP:MA3-064_H3K9me2S10ph_P68431-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2633021

Product Specific Information

MA3-064 detects dimethyl-phospho- histone H3 Lys9 pSer10 in human samples and has successfully been used in western blot and immunoprecipitation applications.

The MA3-064 immunogen is a synthetic peptide conjugated to ovalbumin corresponding to residues 7-20 of Human Histone H3, which is highly conserved across multiple species.

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.