Thermo Fisher Scientific CD148 Monoclonal Antibody (MEM-CD148/05), APC

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL/1x10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

MEM-CD148/05

Immunogen

Human recombinant CD148 (amino acids 1-444) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD148,uniProtId:Q12913-1,ncbiNodeId:9606,antigenRange:1-444,antigenLength:1337,antigenImageFileName:MA5-28590_CD148_Q12913-1_House_mouse.svg,antigenImageFileNamePDP:MA5-28590_CD148_Q12913-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

View additional formats

• Unconjugated
• Biotin
• PE
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745549

Product Specific Information

This antibody recognizes an extracellular epitope of CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.

Recommended dilution is for 100 µL of whole blood or 1x10^6 cells in suspension.

Target Information

CD148 is a highly glycosylated transmembrane protein of up to 250 kDa, containing eight fibronectin type III domains in the extracellular region and one tyrosine phosphatase domain in the cytoplasmic region. Expression is found broadly on fibroblasts, epithelial cells, lymphocytes (B, T and NK cells), and myeloid cells (monocytes and granulocytes). CD148 can dephosphorylate proteins such as LAT and PLC-gamma, and is involved in the downregulation of T cell activation. Conversely, CD148 expression can be upregulated following T cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.