Thermo Fisher Scientific CD56 (NCAM) Monoclonal Antibody (TULY56), APC-eFluor 780, eBioscience

Thermo Fisher Scientific CD56 (NCAM) Monoclonal Antibody (TULY56), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 7 publications

Product Specifications

Additional Formats Available

• Alexa Fluor 660
• Alexa Fluor 700
• APC
• Brilliant UV 395, 563, 661, 737, 805
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• NovaFluor Blue 660-120S, 800
• NovaFluor Red 700, 710, 725
• NovaFluor Yellow 690, 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

This TULY56 monoclonal antibody reacts with human CD56 (Neural Cell Adhesion Molecule, NCAM).
CD56 is a glycosylated transmembrane molecule expressed by neurons and involved in homotypic neural cell adhesion.
In hematopoietic cells, CD56 is expressed on NK cells and a subset of NKT cells.

• TULY56 does not block CMSSB binding, indicating distinct epitopes.
• Performs better after fixation and permeabilization compared to CMSSB.
• Crossreacts with Rhesus macaque.
• Reported application: Flow cytometric analysis.
• Tested by flow cytometry on normal human peripheral blood cells.
• Recommended use: 5 µL (0.25 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
APC-eFluor 780 emits at 780 nm and is excited by the Red laser (633–647 nm). Ensure instrument compatibility.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation; protect vial and samples from light.

Fixation Guidance:
Samples may be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-Step Fix/Lyse Solution (Product #00-5333-57)
  for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.

Excitation: 633–647 nm
Emission: 780 nm
Laser: Red Laser

Target Information

CD56 (NCAM) is a transmembrane glycoprotein of the immunoglobulin family acting as an adhesive molecule.
It is expressed throughout the nervous system (120–180 kDa isoforms) and found on T cells and NK cells.
Functions include cell migration, axonal growth, pathfinding, and synaptic plasticity.

Polysialic modification reduces CD56-mediated adhesion. Through its extracellular domain, CD56 binds laminin and integrins, mediating homophilic and heterophilic interactions.

CD56 is expressed on most neuroectodermal-derived cell lines, tissues, and neoplasms (e.g., retinoblastoma, medulloblastoma, astrocytoma, neuroblastoma).
It serves as a sensitive neuroendocrine marker for neuroendocrine tumors and ovarian granulosa cell tumors.

Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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