Thermo Fisher Scientific DYKDDDDK Tag Monoclonal Antibody (5A8E5), iFluor 488, THE

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1-4 µg/mL

-

Flow Cytometry (Flow)

1-4 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

5A8E5

Immunogen

A synthetic peptide DYKDDDDK coupled to KLH.

Conjugate

iFluor™ 488 iFluor™ 488 iFluor™ 488

View additional formats

• Unconjugated
• Biotin
• FITC
• HRP
• iFluor 555
• iFluor 647
• Request custom conjugation

Excitation/Emission Max

491/516 nm

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 10mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

The Flag tag is a polypeptide of with the amino acid sequence DYKDDDDK amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-Flag tag antibody is a useful tool for the analysis of Flag-tagged proteins.THE™ DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse is THE™ DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.

Target Information

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.