
Thermo Fisher Scientific HA Tag Monoclonal Antibody (5E11D8), iFluor 488, THE
HA 태그 단백질 검출용 iFluor 488 결합 단일클론 항체. ICC/IF 및 유세포분석에 적합. 높은 형광 안정성과 밝기 제공. 단백질 발현 분석 및 정제 연구용. 동결건조 형태로 -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–4 µg/mL |
| Flow Cytometry (Flow) | 1–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E11D8 |
| Immunogen | Synthetic peptide YPYDVPDYA coupled to KLH |
| Conjugate | iFluor™ 488 |
| Excitation / Emission Max | 491 / 516 nm |
| Form | Lyophilized |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 10 mg/mL BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C (preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- FITC
- HRP
- iFluor 647
- Custom conjugation available upon request
Product Specific Information
Reconstitute the lyophilized product with deionized water (or equivalent) to make the antibody concentration of 0.5 mg/mL.
The HA tag is a polypeptide with the amino acid sequence YPYDVPDYA that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-HA tag antibody is a useful tool for the analysis of HA-tagged proteins.
THE™ HA Tag Antibody [iFluor 488], mAb, Mouse is THE™ HA Tag Antibody (A01244) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4–8. It is suitable for detecting the expression level of HA-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye, serving as an excellent alternative to Alexa Fluor 488.
Target Information
HA Tag (hemagglutinin tag) is an epitope tag used to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when specific antibodies are unavailable. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, commonly expressed as a tag to aid in identification and purification of tagged proteins for functional analysis. HA tag antibodies facilitate protein detection and co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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