Thermo Fisher Scientific Phosphotyrosine Antibody Cocktail, THE

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

4 µg/mL

-

Flow Cytometry (Flow)

4 µg/mL

-

ELISA (ELISA)

0.1 µg/mL

-

Immunoprecipitation (IP)

1-4 µg/mL

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG1, kappa/IgG2a, kappa

Class

Cocktail

Type

Antibody

Clone

5E10E1, 18E10D2

Immunogen

Phosphotyrosine containing carrier protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Phosphotyrosine is a tyrosine residue covalently bound to a phosphate via its hydroxyl group. A tyrosine kinase catalyzes the transfer of a phosphate from ATP to a tyrosine residue on the protein substrate. Tyrosine phosphorylation plays a key role in intracellular signaling and cancer development. Many tyrosine kinases are drug targets for different forms of cancer. Phosphotyrosine antibody specifically binds to phosphotyrosine residues. It is a valuablel tool to analyze tyrosine phosphorylation and monitor the activity of tyrosine kinase in high throughput drug discovery. THE™ Phosphotyrosine Antibody (E10) plus recognizes a broadspectrum of phosphotyrosine-containing proteins from numerous species.GenScript THE™ Phosphotyrosine Antibody (E10) plus is an anti-Phosphotyrosine monoclonal antibody (5E10E1) mixed with another anti-Phosphotyrosine monoclonal antibody (18E10D2) at an optimal ratio. The antibodies are produced from a hybridoma resulting from the fusion of SP2/0-Ag14 myeloma and B-lymphocytes harvested from mouse immunized with phosphotyrosine conjugated to KLH.

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.