Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), iFluor 647, THE

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1–4 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 1–4 µg/mL

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• HRP
• iFluor 488
• iFluor 555
• Request custom conjugation

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL.

The V5 tag is a polypeptide of GKPIPNPLLGLDST amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus, C-terminus, or inserted in an internal region of the protein to facilitate detection and purification.

An anti-V5 tag antibody is a useful tool for the analysis of V5-tagged proteins.
THE™ V5 Tag Antibody [iFluor 647], mAb, Mouse is THE™ V5 Tag Antibody (A01724) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3–6.
It is suitable for detecting the expression level of V5-tagged proteins.
iFluor 647 is a bright and photostable fluorescent dye, an excellent alternative to Alexa Fluor 647.

Target Information

Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or poorly immunogenic proteins when specific antibodies are unavailable.

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5).
The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), though a shorter 9 amino acid sequence (IPNPLLGLD) may also be used.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.