Thermo Fisher Scientific V5 Tag Monoclonal Antibody (4C12E11), iFluor 647, THE

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1-4 µg/mL

-

Flow Cytometry (Flow)

1-4 µg/mL

-

Product Specifications

Species Reactivity

Tag

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4C12E11

Immunogen

A synthetic peptide GKPIPNPLLGLDST coupled to KLH

Conjugate

iFluor™ 647 iFluor™ 647 iFluor™ 647

View additional formats

• Unconjugated
• Biotin
• HRP
• iFluor 488
• iFluor 555
• Request custom conjugation

Excitation/Emission Max

656/670 nm

Form

Lyophilized

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 10mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.

The V5 tag is a polypeptide of GKPIPNPLLGLDST amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus, C-terminus or inserted in an internal region of the protein to facilitate detection and purification. An anti-V5 tag antibody is a useful tool for the analysis of V5-tagged proteins. THE™ V5 Tag Antibody [iFluor 647], mAb, Mouse is THE™ V5 Tag Antibody (A01724) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of V5-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.

Target Information

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.