Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 2 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• eFluor 615
• FITC
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The HI98 monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure to varying degrees, while lymphocytes are negative.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

Optical Properties:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1)
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system, and tumor cells. Variants include sialyl-Lewis X and sulphated Lewis X.
High surface expression of Le(x) antigen promotes calcium-dependent self-aggregation, relevant in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used in the diagnosis of Hodgkin’s disease.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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