Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), PE, eBioscience
인간 CD15(Lewis X) 항원을 인식하는 HI98 단클론 항체로, PE 형광 표지되어 유세포분석(Flow Cytometry)에 적합합니다. 5 µL/test로 사용하며, 488–561 nm 여기 파장에서 578 nm 방출. 4°C 암소 보관, 동결 금지.
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- 12-0159-4x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), PE, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HI98 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11218501 |
Available Formats
- Unconjugated
- APC
- Biotin
- eFluor 450
- eFluor 615
- FITC
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
The HI98 monoclonal antibody reacts with human CD15 (Lewis X), a 3-fucosyl-N-acetyllactosamine carbohydrate moiety expressed by granulocytes. Monocytes express this structure to varying degrees, while lymphocytes are negative.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.125 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.
Optical Properties:
- Excitation: 488–561 nm
- Emission: 578 nm
- Compatible Lasers: Blue, Green, Yellow-Green
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1)
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system, and tumor cells. Variants include sialyl-Lewis X and sulphated Lewis X.
High surface expression of Le(x) antigen promotes calcium-dependent self-aggregation, relevant in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used in the diagnosis of Hodgkin’s disease.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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