Thermo Fisher Scientific NOTCH1 Monoclonal Antibody (OTI2E7), TrueMAB
NOTCH1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Human, Mouse, Rat 시료에 반응. IHC(P)에서 1:150 희석으로 사용 가능. 동결건조 형태로 공급되며, PBS 버퍼(8% trehalose) 포함. 연구용으로 신경계 및 세포 분화 관련 연구에 적합.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E7 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2280–2556 of human NOTCH1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The NOTCH gene family encodes transmembrane proteins with multiple cysteine-rich EGF-like repeats in the extracellular region.
Four mammalian NOTCH genes have been identified: Notch1, Notch2, Notch3, and Notch4 (Int-3), which play roles in nervous system differentiation and other developmental processes.
Ligands such as Delta and Serrate interact with Notch1. Mature Notch proteins are heterodimeric receptors derived from cleavage of precursor proteins into extracellular (NEC) and transmembrane (Ntm) subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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