Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor Yellow 660, eBioscience

Applications

• Flow Cytometry (Flow): Tested ≤ 0.2 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 700
• PE / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 702
• Custom conjugation available

Product Specific Information

Description:
The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 forms heterodimers with alpha integrins (CD49a–f) to create VLA-1 through VLA-6 complexes, mediating adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins interact with extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin, and with receptors including VCAM-1 and MadCAM-1.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow cells at ≤ 0.2 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology, engineered for optimized fluorescence performance with reduced spillover and spread.

Optical Properties:

• Excitation: 552 nm
• Emission: 663 nm
• Recommended laser: 561 nm (Yellow)

Strain Note:
NovaFluor Yellow 660 may non-specifically stain B cells in SJL mice, but not in BALB/c or C57BL/6 strains.

Target Information

ITGB1 (Integrin Subunit Beta 1, CD29):
A 110 kDa cell surface glycoprotein expressed by various cells including leukocytes. Forms heterodimers with alpha chains (α1–α6), functioning as VLA integrins. Plays a key role in adhesion, migration, and immune cell trafficking. Expression changes are associated with tumor multidrug resistance and inflammatory responses. Two main isoforms exist: β1A (ubiquitous) and β1D (cardiac and skeletal muscle).

For Research Use Only. Not for diagnostic or resale purposes.

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