Thermo Fisher Scientific CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), NovaFluor Yellow 660, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 0.2 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 700
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110–120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle, and epithelial cells. CD29 binds non-covalently with alpha integrins (CD49a-f, CD51) to form VLA-1 through VLA-6 complexes, mediating adhesion, trafficking, proliferation, and differentiation.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Mouse bone marrow cells, ≤0.2 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining. Add 5 µL per sample or per 100 µL of cell sample.
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures with minimized spillover and spread.
• Excitation: 552 nm; Emission: 663 nm; Laser: 561 nm (Yellow).
• Internal testing shows non-specific B-cell staining in SJL mice, not observed in BALB/c or C57BL/6 strains.

Target Information

ITGB1 (Integrin Subunit Beta 1, CD29) is a 110 kDa cell surface glycoprotein expressed by various cells including leukocytes. It forms heterodimers with at least six alpha chains (α1–α6), determining VLA integrin binding properties. ITGB1 functions in adhesion and migration, and its altered expression is associated with tumor multidrug resistance, immune cell trafficking, and inflammatory responses. Two major isoforms exist: β1A (ubiquitous) and β1D (cardiac/skeletal muscle-specific).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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