Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), NovaFluor Red 755, eBioscience

Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), NovaFluor Red 755, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.6 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. CD1d associates with β2-microglobulin and functionally resembles MHC class II. The 1B1 clone detects CD1d at varying levels on mouse leukocytes and recognizes β2m-associated CD1d.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested by flow cytometric analysis of mouse splenocytes; use ≤ 0.6 µg/test (100 µL final volume).
• Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Each product contains:

1. 1 vial of NovaFluor conjugate
2. 1 vial of CellBlox Plus Blocking Buffer

Compatibility:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI). Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Improved formulation over CellBlox
• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample (10³–10⁸ cells) prior to combining with cells
• For single-color controls: use 5 µL per 100 µL cell sample

Technology:
NovaFluor conjugates utilize Phiton™ technology based on novel nucleic acid dye structures, engineered for controlled fluorescent signatures minimizing spillover and spread.

Excitation / Emission (NovaFluor Red 755):
636 nm / 756 nm; Laser: 633–640 nm (Red)

Target Information

CD1d is a heavy chain associated with β2-microglobulin on cortical thymocytes. It is the sole group 2 member of the CD1 family, structurally related to MHC proteins, and forms heterodimers with β2-microglobulin. CD1 proteins present lipid and glycolipid antigens to T cells. The human genome contains five CD1 genes on chromosome 1. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지