Thermo Fisher Scientific Nestin Monoclonal Antibody (Rat-401 (Rat401 (4D4))), eBioscience

Applications and Tested Dilutions

Product Specifications

Product Specific Information

The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells. Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells. Nestin expression is also found in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells. Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers. Upon differentiation, Nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP.

Applications Reported:
This Rat-401 (Rat401 (4D4)) antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and immunocytochemistry.

Applications Tested:
This Rat-401 antibody has been tested by immunofluorescent staining of paraformaldehyde-fixed and permeabilized cells and by immunohistochemistry of formalin-fixed paraffin-embedded mouse tissue using low or high pH antigen retrieval. Recommended concentration ≤10 µg/mL. Antibody should be titrated for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Highly specific antibodies to human Nestin can help characterize progenitor cells differentiating into distinct lineages, enhance therapeutic potential of neural stem/progenitor cells in CNS disease treatment, and identify neuroepithelial tumor cells. Nestin expression is observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.