Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), eBioscience
Mouse 및 Human T-bet 단백질을 인식하는 eBio4B10 단일클론 항체로, Th1 세포 발달 연구에 적합. WB, IHC, ICC, IP 등 다양한 응용 가능. 고순도(>90%) 및 낮은 응집도(<10%), 액상 형태로 PBS buffer에 보존.
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- 14-5825-xx (4개 옵션)
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Thermo Fisher Scientific T-bet Monoclonal Antibody (eBio4B10 (4B10)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 8 publications |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 5 publications |
| Flow Cytometry (Flow) | - | View 99 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | - | View 2 publications |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rhesus monkey |
| Published Species | Bat, Human, Mouse, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio4B10 (4B10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865145 |
Available Conjugated Formats
- Alexa Fluor 488
- Alexa Fluor 660
- APC
- eFluor 450
- eFluor 660
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The eBio4B10 monoclonal antibody reacts with mouse and human T-bet.
T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage.
T-bet expression is induced by IFN-γ and regulates IFN-γ, IL-12Rβ, and IL-2 expression through chromatin remodeling.
It also plays a role in class-switch recombination in B-cells.
Applications Reported: Immunohistochemical staining (FFPE tissue, citrate retrieval), immunoprecipitation, and western blotting.
Applications Tested: Western blot analysis of Th1-polarized mouse CD4+ T cells (recommended starting dilution: 2 µg/mL).
Purity: >90% by SDS-PAGE.
Aggregation: <10% by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
T-bet (TBX21) is a T-cell specific transcription factor containing a T-box DNA-binding domain.
It controls expression of the Th1 cytokine interferon-gamma and regulates Th1 vs Th17 differentiation.
T-bet cooperates with Stat4 to program chromatin architecture for Th1 gene expression.
Genetic variations in T-bet are associated with aspirin-induced asthma and nasal polyps.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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