Thermo Fisher Scientific CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 6 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• APC-eFluor 780
• Brilliant UV 496 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue / Red / Yellow Series
• PE / PE-Cyanine5 / PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600
• Request custom conjugation

Product Specific Information

Description:
The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells. It binds glycosylated, fucosylated, and sulfated glycoproteins such as CD34, glycam-1, and MAdCAM-1, mediating lymphocyte rolling on activated endothelium and homing to high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Recommended usage: 5 µL (0.125 µg) per test in a final volume of 100 µL. Cell number can range from 10⁵ to 10⁸ cells/test.

Fluorophore Information:
PE-eFluor® 610 can be excited with laser lines from 488–561 nm and emits at 607 nm. Recommended filter: 610/20 band pass (equivalent to PE-Texas Red®). Ensure instrument compatibility.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be determined empirically.

Excitation / Emission:
488–561 nm / 607 nm
Laser Compatibility: Blue, Green, Yellow-Green Lasers
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Human CD62L (L-Selectin) is a 74–95 kDa glycoprotein of the selectin family of adhesion receptors. It comprises:

• N-terminal C-type lectin binding domain
• EGF-like domain
• Two short consensus repeat (SCR) sequences
• Spacer, transmembrane, and cytoplasmic regions

CD62L is constitutively expressed on most leukocytes including lymphocytes, monocytes, and polymorphonuclear cells, except a subset of memory T cells.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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