Thermo Fisher Scientific CD68 Monoclonal Antibody (FA-11), PE-eFluor 610, eBioscience
Mouse CD68 단백질을 인식하는 FA-11 클론 단일클론 항체로, PE-eFluor 610 형광체가 결합된 제품입니다. Flow cytometry 및 면역염색에 적합하며, 488–561 nm 레이저로 여기됩니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 61-0681-8x (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD68 Monoclonal Antibody (FA-11), PE-eFluor 610, eBioscience
Thermo Fisher Scientific CD68 Monoclonal Antibody (FA-11), PE-eFluor 610, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/test | - |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FA-11 |
| Conjugate | PE-eFluor™ 610 |
| Excitation / Emission Max | 565 / 606 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2802374 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC-eFluor 780
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 786
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
Description:
FA-11 monoclonal antibody reacts with mouse CD68 (macrosialin), a heavily O- and N-glycosylated type I integral membrane protein of the sialomucin family. CD68 is mainly localized in late endosomes and lysosomes, with a smaller fraction at the cell surface. It serves as a pan-macrophage marker but can also be expressed in Langerhans and dendritic cells. CD68 binds oxidized LDL and participates in phagocytosis.
Applications Reported:
Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Validated for intracellular staining of mouse bone marrow-derived macrophages using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤1.0 µg/test (100 µL final volume). Optimize empirically for best performance.
Fluorochrome Information:
PE-eFluor 610 is excitable by 488–561 nm laser lines and emits at 607 nm. Recommended filter: 610/20 band pass (PE-Texas Red equivalent). Ensure instrument compatibility.
Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.
Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) up to 3 days at 4°C in dark with minimal brightness loss.
Excitation / Emission / Laser:
488–561 nm excitation; 607 nm emission; compatible with Blue, Green, and Yellow-Green lasers.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein mainly expressed in lysosomes of monocytes and macrophages, and to a lesser extent in dendritic cells and granulocytes. It plays roles in phagocytosis and cell-pathogen interactions. CD68 expression is elevated in certain malignancies, including AML and histiocytic disorders.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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