Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), NovaFluor Blue 690, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats Available

• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 480
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

Description:
The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein-linked chemokine receptor binding CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a role in CD4 T cell responses to grafts; knockout mice show compromised allograft rejection. Expression is found on NK cells, subsets of T lymphocytes, and Th1-polarized cells. The CXCR3-173 antibody affects chemotaxis in response to ligands; ligand presence eliminates staining. In vivo addition delays cardiac and pancreatic islet allograft rejection.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes at ≤0.25 µg/test (100 µL final volume). Optimal titration is recommended per assay.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• NovaFluor conjugates now ship with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates use Phiton™ technology with engineered fluorescent signatures optimized for spillover and spread control.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily, characterized by a single amino acid separating the first two conserved cysteine residues. Binding induces leukocyte trafficking responses such as integrin activation and chemotaxis. Expression is prominent in effector/memory T cells and inflamed tissues, suggesting roles in inflammatory cell recruitment. This receptor is a potential target for small-molecule antagonists in inflammatory disease treatment. Multiple transcript variants exist.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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