Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 510, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q, and/or H-2L^q. Binding to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes; use ≤1 µg/test (100 µL final volume, 10^5–10^8 cells/test). Optimize titration for best results.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails before adding cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10^3–10^8 cells).

NovaFluor Conjugate Technology:
Based on Phiton™ technology utilizing novel nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

Excitation / Emission / Laser:
496 nm / 511 nm / 488 nm (Blue laser)

Target Information

This protein is an MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8+ T cells.
HLA and MHC antibodies are crucial in immunopeptidomics for identifying and characterizing neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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