Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.6 µg/test

Product Specifications

Additional Formats

• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 480
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation available

Product Specific Information

The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein-linked chemokine receptor binding CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC). CD183 plays a role in CD4 T cell responses to grafts, with knockout mice showing compromised allograft rejection. Expression occurs on NK cells, subsets of T lymphocytes, Tregs, and Th1-polarized cells. The antibody affects chemotaxis in response to ligand, and ligand presence eliminates staining. In vivo addition delays cardiac and pancreatic islet allograft rejection.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; optimal at ≤0.25 µg per test in 100 µL volume (10⁵–10⁸ cells/test). Titrate for best performance.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample (regardless of NovaFluor number) before combining with cells.
• For single-color controls, add 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Conjugate Technology:
Based on Phiton™ technology utilizing nucleic acid dye structures engineered for optimized fluorescent signatures minimizing spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These chemokines belong to the CXC subfamily, inducing cellular responses like integrin activation, cytoskeletal rearrangement, and chemotaxis. Inhibition by Bordetella pertussis toxin indicates coupling with Gi-subclass G proteins. Expression is high in effector/memory T cells and inflamed tissues, suggesting a role in inflammatory cell recruitment. The receptor is a potential target for small-molecule antagonists in inflammatory disease treatment. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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