Thermo Fisher Scientific Actin Monoclonal Antibody (A2E1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A2E1

Immunogen

Synthetic peptide corresponding to N terminal of Human Alpha actin (smooth muscle, skeletal muscle, cardiac muscle) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Actin,uniProtId:P68133-1,ncbiNodeId:9606,antigenRange:1,antigenLength:377,antigenImageFileName:MA5-42536_Actin_P68133-1_House_mouse.svg,antigenImageFileNamePDP:MA5-42536_Actin_P68133-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2911677

Product Specific Information

Positive Control: Rat heart tissue lysate, mouse smooth muscle tissue lysate, human skeletal muscle tissue lysate.

Subcellular Location: Cytoplasm, Cytoskeleton

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.