Thermo Fisher Scientific FEN1 Monoclonal Antibody (A1B12)
FEN1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, Flow Cytometry에 적합. Human, Mouse, Rat 반응성. Protein G 정제, 2 mg/mL 농도, TBS buffer. DNA 복제 및 수선 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1B12 |
| Immunogen | Recombinant protein within human FEN1 aa 150–380 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911675 |
Product Specific Information
- Positive Control: MCF-7, K562, Daudi, PC-12, NIH/3T3 cell lysates; human tonsil, liver carcinoma, and breast carcinoma tissues.
- Subcellular Location: Nucleolus, nucleoplasm, mitochondrion.
Target Information
FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein functioning as an endonuclease that cleaves branched DNA structures containing single-stranded 5′ flaps or as an exonuclease on DNA nicks and gaps. Its activities are stimulated by association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 plays critical roles in DNA replication and repair and is essential in cell-free DNA replication systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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