Thermo Fisher Scientific Erythropoietin (EPO) Recombinant Rabbit Monoclonal Antibody (EPO, 3793R)
Erythropoietin(EPO)에 특이적인 recombinant rabbit monoclonal antibody. 인체 시료 반응성, HEK293 발현 시스템 기반. IHC(P)에서 2–4 µg/mL로 사용 가능. 단백질 A/G 정제 및 PBS(pH 7.4) buffer, 보존제 없음. 연구용 전용.
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Applications
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 2–4 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | EPO, 3793R |
| Immunogen | Recombinant fragment (around aa 28–162) of human EPO protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C (if preferred) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Tested in direct ELISA (use 2–4 µg/mL for coating).
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating in 10 mM Tris buffer with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Erythropoietin (EPO) is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. It induces the final maturation of partially differentiated erythroid cells and promotes production of red cell-specific proteins such as hemoglobin. The EPO receptor (EPOR) is highly expressed on precursor cells and decreases with maturation.
In fetuses, hepatocytes are the main source of EPO, while in adults, interstitial kidney cells are predominant. EPO expression increases during hypoxia, and low expression can occur in lung and brain tissue. Recent findings suggest EPO may also protect neuronal tissue and aid in repair mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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