Thermo Fisher Scientific Erythropoietin (EPO) Recombinant Rabbit Monoclonal Antibody (EPO, 3793R)

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 2–4 µg/mL

Product Specifications

Product Specific Information

• Tested in direct ELISA (use 2–4 µg/mL for coating).
• For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating in 10 mM Tris buffer with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Erythropoietin (EPO) is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. It induces the final maturation of partially differentiated erythroid cells and promotes production of red cell-specific proteins such as hemoglobin. The EPO receptor (EPOR) is highly expressed on precursor cells and decreases with maturation.
In fetuses, hepatocytes are the main source of EPO, while in adults, interstitial kidney cells are predominant. EPO expression increases during hypoxia, and low expression can occur in lung and brain tissue. Recent findings suggest EPO may also protect neuronal tissue and aid in repair mechanisms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.