Thermo Fisher Scientific Erythropoietin (EPO) Recombinant Mouse Monoclonal Antibody (rEPO, 1367)

Applications

Tested Application

• Immunohistochemistry (Paraffin) (IHC (P)): 2–4 µg/mL

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Erythropoietin (EPO) is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. It is not responsible for early lineage commitment but induces final maturation of partially differentiated erythroid cells and production of red cell-specific proteins such as hemoglobin.
The EPO receptor (EPOR) is highly expressed on precursor cells, with expression diminishing during maturation. Hepatocytes are the main source of EPO in the fetus, while interstitial kidney cells become the primary source after birth.
EPO expression increases during hypoxia and has been detected at low levels in lung and brain tissue under such conditions. Recent studies suggest EPO may also protect neuronal tissue and aid in repair processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.