Thermo Fisher Scientific Erythropoietin (EPO) Recombinant Mouse Monoclonal Antibody (rEPO, 1367)
Erythropoietin(EPO)에 특이적인 마우스 단클론 항체로, IHC(P) 및 ELISA에 적합합니다. HEK293 세포에서 발현된 재조합 항체이며, Protein A/G로 정제되었습니다. PBS(pH 7.4) 용액 상태로 제공되며, 보존제가 포함되어 있지 않습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rEPO, 1367 |
| Immunogen | Recombinant fragment (around aa 28–162) of human EPO protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Tested in direct ELISA (use antibody at 2–4 µg/mL for coating).
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris Buffer with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Erythropoietin (EPO) is a growth factor essential for the survival, proliferation, and differentiation of erythroid precursors. It induces final maturation of erythroid cells and production of red cell-specific proteins such as hemoglobin. The EPO receptor (EPOR) is highly expressed on precursor cells and decreases with maturation.
In the fetus, hepatocytes are the main source of EPO, while in adults, interstitial kidney cells are predominant. EPO expression increases under hypoxic conditions and may also play roles in neuronal tissue protection and repair.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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