Gibco™

DMEM/F-12, powder, HEPES

DMEM/F-12 (Dulbecco`s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different자세히 알아보기

DMEM/F-12 (Dulbecco`s Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With	Without
• L-glutamine	• Sodium Bicarbonate
• HEPES
• Phenol Red

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids, and vitamins with F-12`s wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.

사양

세포주MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts

분류Animal Origin-free

형태Powder

제품 유형DMEM (Dulbecco`s Modified Eagle Medium)/F-12

Serum LevelStandard Serum Supplementation

유통 기한36 Months From Date of Manufacture

멸균Sterile-filtered

첨가제 포함High Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate

첨가제 없음No Sodium Bicarbonate

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량10 x 1 L

배송 조건Room Temperature

Unit SizeEach