Gibco™

DMEM, high glucose, HEPES

Dulbecco`s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.자세히 알아보기

Dulbecco`s Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• Sodium Pyruvate
• L-glutamine
• HEPES
• Phenol Red

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle`s Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ DMEM is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers an identical Gibco™ DMEM product made in our Grand Island facility (12430-062). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

사양

세포주HeLa, 293, Cos-7, and PC-12

세포 유형Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells

분류Animal Origin-free

농도1 X

형태Liquid

제품 유형DMEM (Dulbecco`s Modified Eagle Medium)

Serum LevelStandard Serum Supplementation

유통 기한12 Months From Date of Manufacture

멸균Sterile-filtered

Sterilization MethodSterile-filtered

첨가제 포함High Glucose, Glutamine, HEPES, Phenol Red

첨가제 없음No Sodium Pyruvate

용도(애플리케이션)Mammalian cell culture

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량1000 mL

배송 조건Room Temperature

Unit SizeEach