Thermo Fisher Scientific CD169 (Siglec-1) Monoclonal Antibody (SER-4), PE, eBioscience

Applications

Immunohistochemistry (Frozen) (IHC (F))

• Tested Dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested Dilution: 1 µg/test
• Publications: View 5 publications

Product Specifications

Additional Formats

• Alexa Fluor 488
• APC-eFluor 780
• eFluor 660
• Request custom conjugation

Product Specific Information

The SER-4 monoclonal antibody reacts with mouse CD169 (Sialoadhesin, Siglec-1), a 185-kDa member of the Siglec family that recognizes sialylated glycans. CD169 is expressed on macrophage subsets in the spleen, liver, lymph node, and bone marrow, and can also be detected on monocytes.
It mediates cell-cell adhesion and internalization of sialylated viruses and bacteria, enabling CD169⁺ macrophages to present lipid antigens to iNKT cells. Interaction with regulatory T cells can inhibit their proliferation.

• Reported Activity: Antagonist
• Reported Applications: Flow cytometry
• Tested Applications: Flow cytometric analysis of mouse bone marrow cells (≤1 µg/test)
• Recommended: Titrate antibody for optimal performance
• Excitation: 488–561 nm; Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Siglecs are membrane-bound lectins (immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Members include sialoadhesin, CD22, and CD33.
Sialoadhesin is a macrophage-specific adhesion molecule expressed on activated macrophages in chronic inflammation and tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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