Thermo Fisher Scientific VSIG4 Monoclonal Antibody (NLA14), PE, eBioscience

Thermo Fisher Scientific VSIG4 Monoclonal Antibody (NLA14), PE, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: View 5 publications

Product Specifications

Additional Formats:

• Alexa Fluor 488
• APC
• PE-Cyanine7
• PE-eFluor 610
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

This NLA14 monoclonal antibody recognizes mouse V-Set and Immunoglobulin domain containing 4 (VSIG4), also known as Complement Receptor of the Immunoglobulin superfamily (CRIg) or Z39Ig.
VSIG4 is a type I transmembrane glycoprotein structurally related to the B7 family of immune regulatory proteins. It contains one complete V-type Ig domain and one truncated C-type Ig domain.

VSIG4 is expressed exclusively on tissue resident and tumor-infiltrating macrophages. It binds complement components C3b and iC3b, inhibiting the alternative complement pathway and facilitating phagocytosis of complement-opsonized pathogens. Additionally, VSIG4 suppresses T cell activation, proliferation, and IL-2 production, contributing to peripheral T cell tolerance and inflammation suppression.

Expression of VSIG4 on tumor-infiltrating macrophages suggests a role in immune evasion. Pro-inflammatory stimuli such as TNF and LPS down-regulate VSIG4 expression. Peritoneal macrophages in Balb/c mice express higher VSIG4 levels than those in C57Bl/6 or Swiss Webster mice.

The NLA14 antibody recognizes VSIG4 on formaldehyde-fixed and permeabilized cells. It does not block VSIG4–T cell ligand interactions and does not cross-react with rat or human VSIG4.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of resident peritoneal macrophages

• Recommended usage ≤ 0.5 µg/test (100 µL final volume)
• Cell count range: 10⁵–10⁸ cells/test
• Optimize antibody titration for best performance

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers

Target Information

T cell activation by APCs is regulated by B7 family members. VSIG4 is a strong negative regulator of murine and human T cell proliferation and IL-2 production.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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