Thermo Fisher Scientific NOTCH3 Monoclonal Antibody (HMN3-133), PE, eBioscience

Applications and Tested Dilution

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Product Specifications

Additional Formats

• APC
• Request custom conjugation

Product Specific Information

This HMN3-133 monoclonal antibody reacts with mouse Notch3, one of four members of the Notch family of receptors. Notch receptors are 300-kDa single-pass transmembrane proteins. The extracellular domain contains epidermal growth factor-like repeats for ligand binding, while the intracellular domain participates in cell signaling.

Upon binding its membrane-bound ligand (Delta or Jagged), the Notch receptor undergoes proteolytic cleavage by ADAM-family metalloproteases and gamma-secretase. The released Notch intracellular domain (NICD) translocates into the nucleus, associates with RBP-J, recruits co-activators, and activates transcription.

Notch3 expression occurs in regulatory T cells, thymocyte subsets (CD4-CD8-, CD8SP), vascular smooth muscle, and the central nervous system. It plays a role in hematopoiesis, T cell lineage commitment, and thymocyte development, particularly in the DN3 (CD4-CD8-CD25+CD44-) stage. Notch3 may regulate the pre-TCR checkpoint via NFkappaB and E2A signaling. Overexpression of Notch3 has been implicated in leukemia.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse thymocytes or transfected cells at ≤0.5 µg/test.
A test is defined as the amount of antibody that stains cells in 100 µL volume, typically 10⁵–10⁸ cells/test.
Careful titration is recommended for optimal performance.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

NOTCH3 is a human homologue of the Drosophila Notch type I membrane protein. In Drosophila, Notch-ligand interactions (delta, serrate) regulate neural development. Human homologues of these ligands have been identified. NOTCH3 functions as a receptor for Jagged1, Jagged2, and Delta1, regulating cell-fate determination. Mutations in NOTCH3 are associated with CADASIL (Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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