Thermo Fisher Scientific TIGIT Monoclonal Antibody (GIGD7), Brilliant Violet 480, eBioscience

Thermo Fisher Scientific TIGIT Monoclonal Antibody (GIGD7), Brilliant Violet 480

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Brilliant Violet 421
• Brilliant Violet 650
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 702
• Request custom conjugation

Product Specific Information

The GIGD7 monoclonal antibody reacts with mouse TIGIT (T cell immunoreceptor with Ig and ITIM domains), a 26 kDa protein in the poliovirus receptor (PVR) family. In mice, TIGIT is expressed in follicular T helper cells; in humans, it is found on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ and CD8+ T cells, but not on B cells or naive CD4+ T cells. TIGIT expression increases upon T cell activation.

TIGIT interacts with members of the PVR and PVR-like families, including CD155, mediating interactions between NK/T cells and antigen-presenting cells, fibroblasts, and endothelial cells expressing PVR proteins.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes; ≤0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.
• Fluorochrome Information: Brilliant Violet™ 480 emits at 479 nm, suitable for cytometers with violet (405 nm) laser.
• Compatibility: Not compatible with methanol-based fixation.
• Recommended Buffers: Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions when using multiple polymer dye-conjugated antibodies.

Excitation: 440 nm
Emission: 479 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

TIGIT binds with high affinity to PVR, leading to increased IL10 secretion, decreased IL12B secretion, and suppression of T cell activation through promotion of mature immunoregulatory dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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