Thermo Fisher Scientific Phospho-Paxillin (Thr538) Polyclonal Antibody
인산화 Paxillin(Thr538)을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, 인간·생쥐·랫드 반응성을 보입니다. Western blot에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 -20°C 보관하며, 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Phospho-Paxillin (Thr538) Polyclonal Antibody
Applications
- Western Blot (WB)
Tested Dilution: 1:200
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-Paxillin (Thr-538) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 538 from human paxillin a. This sequence is highly conserved in rat and mouse paxillin and is also found in the other paxillin isoforms (b & g). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942967 |
Product Specific Information
This antibody detects a 68 kDa protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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