Thermo Fisher Scientific Phospho-Paxillin (Ser178) Polyclonal Antibody
EGF 처리된 A431 세포에서 인산화된 Paxillin(Ser178)을 검출하는 항체. Western blot 및 동결 조직면역염색에 적합. 인간, 생쥐, 랫드 반응성. 항원 친화 크로마토그래피로 정제된 액상 폴리클로날 항체. 연구용 전용.
- 카탈로그번호
- PA5143738
- 판매단위
- pk
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Thermo Fisher Scientific Phospho-Paxillin (Ser178) Polyclonal Antibody
Applications and Tested Dilution
- Western Blot (WB): 1:1,000
- Immunohistochemistry (Frozen) (IHC (F)): 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-Paxillin (Ser-178) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. Highly conserved in rat and mouse. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942966 |
Product Specific Information
This antibody detects a 68 kDa protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of EGF-treated A431 cells, but not in control A431 cells. Similar results were observed in calyculin A-treated human A431 and aortic endothelial cells.
Target Information
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. Its tyrosine phosphorylation is influenced by conditions affecting cell-cell adhesion. Paxillin interacts tightly with FAK and Crk through its SH2 domain, independent of the extracellular matrix. Initially discovered in fibroblasts, paxillin phosphorylation also plays an important role during neurite extension and differentiation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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