Thermo Fisher Scientific Phospho-p130Cas (Tyr762) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-p130 Cas (Tyr-762) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 762 in the Src-binding domain of rat p130 Cas. This rat sequence is the same as Tyr-664 in human p130 Cas and Tyr-668 in mouse p130 Cas. This site is also highly conserved in Cas-L (HEF1, Nedd9).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942962

Product Specific Information

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-751) peptide before affinity purification using phospho-p130 Cas (Tyr-762) peptide (without carrier). The antibody detects a 130 kDa* protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells.

Target Information

The p130Cas (Cas for Crk-associated substrate) is a common cellular target of phosphorylation signal via v-Crk and v-Src oncoproteins. p130Cas has a unique structure that contains a Src homology (SH)-3 domain followed by multiple YXXP motifs and a proline-rich regionp130Cas is implicated in a variety of biological processes including cell adhesion, cell migration, growth factor stimulation, cytokine receptor engagement and bacterial infection. Physiological functions of p130Cas include cardiovascular development, actin filament assembly and Src-induced cell transformation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.