Thermo Fisher Scientific Phospho-PDPK1 (Ser241) Polyclonal Antibody
Phospho-PDPK1 (Ser241)을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Frozen)에 적합. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. PDK1의 활성 조절 연구 및 신호전달 기전 분석에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-PDK1 (Ser-241) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 241 in human PDK1. This sequence is highly conserved in rat and mouse PDK1. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942969 |
Product Specific Information
The purified antibody detects a 65 kDa* protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human MDA-MB-435 cells treated with EGF, but not in untreated cells.
PDK1 (Ser-241) reactivity was also observed by immunohistochemistry in human breast carcinoma tissue.
Target Information
PDPK1 (3-Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases and activates conventional PKC and PKC zeta through phosphorylation of threonine residues in the activation loop.
It also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate.
Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), leading to dephosphorylation and activation of glycogen synthase and stimulation of glycogen synthesis.
Because of its role in insulin-induced glycogen synthesis and PKC activation, PDPK1 is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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