Thermo Fisher Scientific CD68 Monoclonal Antibody (eBioY1/82A (Y1/82A)), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 647
• Biotin
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• Custom conjugation available upon request

Product Specific Information

The eBioY1/82A antibody reacts with CD68, a member of the sialomucin family closely related to lysosomal-associated membrane proteins (LAMPs) and scavenger receptors. CD68 is primarily an intracellular protein localized in late endosomes but also appears on the surface of myeloid-derived cells such as monocytes, macrophages, dendritic cells, neutrophils, basophils, mast cells, and certain hematopoietic progenitor cells.

The function of CD68 is not fully understood, but its structure suggests a role in antigen processing or presentation and in protecting lysosomal membranes from hydrolytic enzymes. Expression has also been observed in activated T cells and subsets of B lymphocytes.

Staining Characteristics:

• Surface staining: B cells and a minor subset of monocytes
• Intracellular staining: ~50% of B cells and most monocytes

Applications Reported:

• Flow cytometric analysis
• Immunohistology of frozen tissue sections

Applications Tested:

• Intracellular staining and flow cytometry (≤1 µg/test)
• A test is defined as the amount of antibody used to stain cells in a 100 µL volume (10⁵–10⁸ cells/test)
• Optimal titration recommended for specific assays

Purity and Quality:

• Purity: >90% (SDS-PAGE)
• Aggregation: <10% (HPLC)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed in lysosomes of monocytes and macrophages, and to a lesser extent in dendritic cells and granulocytes. It plays a role in phagocytic activity, lysosomal metabolism, and cell interactions. CD68 expression is also observed in interdigitating reticulum cells, histiocytic lymphomas, AML, and granulocytic sarcomas. Elevated CD68 levels have been reported in CD34+ cells across various malignancies, including acute myeloid leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.