Thermo Fisher Scientific RUNX2 Recombinant Rabbit Monoclonal Antibody (7O7J4)
RUNX2 단백질을 인식하는 재조합 토끼 단클론 항체로, 인간 및 생쥐 시료에 반응합니다. Western blot, ELISA, IP 등 다양한 응용에 사용 가능하며, HEK293 발현 시스템 기반으로 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific RUNX2 Recombinant Rabbit Monoclonal Antibody (7O7J4)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 400–600 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 7O7J4 |
| Immunogen | Recombinant fusion protein containing amino acids 336–439 of human RUNX2 (Q13950) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911789 |
Product Specific Information
Positive test controls include: U2OS, PC-3, U-937, Mouse testis, Mouse thymus.
Immunogen sequence:
PRRISDDDTA TSDFCLWPST LSKKSQAGAS ELGPFSDPRQ FPSISSLTES RFSNPRMHYP ATFTYTPPVT SGMSLGMSAT THYHTYLPPP YPGSSQSQSG PFQT
Target Information
RUNX2 is a nuclear protein belonging to the RUNX family of transcription factors, characterized by a Runt DNA-binding domain. It is essential for both membranous and endochondral bone formation, regulating osteoblastic differentiation and skeletal morphogenesis. RUNX2 functions as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression, binding DNA as a monomer or as part of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and cooperates with DLX5 or related factors to activate osteoblast-specific gene expression. Mutations in RUNX2 can lead to bone development disorders such as cleidocranial dysplasia (CCD).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지: MA5-42648_RUNX2_Q13950-1_Rabbit.svg, MA5-42648_RUNX2_Q13950-1_Rabbit_PDP.jpeg)
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