Thermo Fisher Scientific IL-16 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
PDLNSSTDSA ASASAASDVS VESTAEATVC TVTLEKMSAG LGFSLEGGKG SLHGDKPLTI NRIFKGAASE QSETVQPGDE ILQLGGTAMQ GLTRFEAWNI IKALPDGPVT IVIRRKSLQS KETTAAGDS.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-16. Anti-Human IL-16-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human IL-16 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-16 (500-P06) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-16.

Western Blot:
To detect hIL-16 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hIL-16 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P06BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by IL-16 is a pleiotropic cytokine functioning as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process is mediated by CD4. IL-16 undergoes proteolytic processing to yield two functional proteins:

• The C-terminal peptide functions as the cytokine.
• The N-terminal product may play a role in cell cycle control.

Caspase 3 is involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.