Thermo Fisher Scientific IL-16 Polyclonal Antibody, PeproTech

Thermo Fisher Scientific IL-16 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
PDLNSSTDSA ASASAASDVS VESTAEATVC TVTLEKMSAG LGFSLEGGKG SLHGDKPLTI NRIFKGAASE QSETVQPGDE ILQLGGTAMQ GLTRFEAWNI IKALPDGPVT IVIRRKSLQS KETTAAGDS

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-16. Anti-Human IL-16-specific antibody purified by affinity chromatography employing an immobilized Human IL-16 matrix.

Sandwich ELISA:
To detect Human IL-16 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human IL-16 (500-P06Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-16.

Western Blot:
To detect Human IL-16 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, detection limit for Recombinant Human IL-16 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

The protein encoded by IL-16 is a pleiotropic cytokine functioning as:

• Chemoattractant
• Modulator of T cell activation
• Inhibitor of HIV replication

Signaling is mediated by CD4. IL-16 undergoes proteolytic processing to yield two functional proteins:

• Secreted C-terminal peptide (cytokine function)
• N-terminal product (cell cycle control)

Caspase 3 is involved in proteolytic processing. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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