Thermo Fisher Scientific IL-16 Polyclonal Antibody, PeproTech
인간 IL-16 단백질을 표적으로 하는 Rabbit Polyclonal Antibody. Western blot, ELISA, 중화시험 및 in vitro assay에 사용 가능. 고순도 항원 친화 크로마토그래피로 정제된 무보존제 제품으로 연구용에 적합.
- 카탈로그번호
- 500-P06-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-16 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific IL-16 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.5–2.0 µg/mL | - |
| Neutralization (Neu) | - | View 1 publication |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-16 (129 a.a.) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929165 |
Product Specific Information
AA Sequence of Recombinant Protein:
PDLNSSTDSA ASASAASDVS VESTAEATVC TVTLEKMSAG LGFSLEGGKG SLHGDKPLTI NRIFKGAASE QSETVQPGDE ILQLGGTAMQ GLTRFEAWNI IKALPDGPVT IVIRRKSLQS KETTAAGDS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-16. Anti-Human IL-16-specific antibody purified by affinity chromatography employing an immobilized Human IL-16 matrix.
Sandwich ELISA:
To detect Human IL-16 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Human IL-16 (500-P06Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-16.
Western Blot:
To detect Human IL-16 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, detection limit for Recombinant Human IL-16 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
The protein encoded by IL-16 is a pleiotropic cytokine functioning as:
- Chemoattractant
- Modulator of T cell activation
- Inhibitor of HIV replication
Signaling is mediated by CD4. IL-16 undergoes proteolytic processing to yield two functional proteins:
- Secreted C-terminal peptide (cytokine function)
- N-terminal product (cell cycle control)
Caspase 3 is involved in proteolytic processing. Alternate splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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