Thermo Fisher Scientific IL-17A Polyclonal Antibody, PeproTech
Human IL-17A를 검출하기 위한 Rabbit Polyclonal Antibody로, WB, IHC, ELISA 등에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 동결건조 형태로 -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | – | View 2 publications |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Immunostaining (IS) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-17A |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929171 |
Product Specific Information
Amino Acid Sequence (Recombinant Protein):
MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17A. Anti-Human IL-17A-specific antibody was purified by affinity chromatography using an immobilized Human IL-17 matrix.
Sandwich ELISA:
To detect Human IL-17A by sandwich ELISA (100 µL/well), use 0.5–2.0 µg/mL of this antibody. In conjunction with PeproTech Biotinylated Anti-Human IL-17A (500-P07Bt) as detection antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Human IL-17A.
Western Blot:
For detection of Human IL-17A by Western Blot, use 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Human IL-17A is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in conditions such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer (136 amino acids) belonging to the IL-17 family (IL-17A–F) and signals via the IL-17 receptor (IL-17R/CDw217).
It induces expression of ICAM-1 in fibroblasts and stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. IL-17A also supports proliferation and differentiation of CD34+ hematopoietic progenitors into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating cross-species receptor recognition.
IL-17A regulates NF-κB and MAPK pathways, enhances IL-6 and COX-2 expression, and promotes nitric oxide production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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