Thermo Fisher Scientific beta-3 Tubulin Monoclonal Antibody (2G10-TB3), Alexa Fluor 488, eBioscience
신경세포 특이적 베타-3 튜불린을 인식하는 단클론 항체로, Alexa Fluor 488 형광 표지. 면역조직화학, 면역세포화학, 유세포분석 등 다양한 응용에 적합. 인간, 마우스, 랫, 소 반응성. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific beta-3 Tubulin Monoclonal Antibody (2G10-TB3), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 4 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 488 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2G10-TB3 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574420 |
Additional Formats
- Unconjugated
- eFluor 570
- eFluor 660
- Request custom conjugation
Product Specific Information
The 2G10 monoclonal antibody recognizes beta tubulin class III, a member of the tubulin family forming microtubules in the cytoskeletal network. Beta tubulin class III expression is specific to neuronal cells in adult mammals and transiently expressed during fetal development in glial and neuronal precursors. It serves as a structural marker for developing and mature neural cells.
Applications Reported: Suitable for immunohistochemistry (frozen and paraffin sections), microscopy, and immunocytochemistry.
Applications Tested: Validated for immunocytochemistry of formaldehyde-fixed and permeabilized cells at ≤10 µg/mL. Optimal dilution should be determined experimentally.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
BetaIII-tubulin is a neuronal marker and an early indicator of neuronal differentiation. It is used as a probe for neuronal cells and tumors of neuronal origin. Although predominantly expressed in neuronal cells, it is also found in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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