Thermo Fisher Scientific ATP5H Monoclonal Antibody (6B12)
ATP5H 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF 등에 사용 가능. 인간, 마우스, 비인간 영장류, 랫트 반응성. 동결 건조 형태로 제공되며, 고순도 친화 크로마토그래피 정제. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6B12 |
| Immunogen | E.coli-derived human ATP5H recombinant protein (Position: A2–L161). Human ATP5H shares 81% and 78% amino acid sequence identity with mouse and rat ATP5H, respectively. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2884069 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation.
ATP synthase consists of two linked multi-subunit complexes:
- F1 (soluble catalytic core)
- Fo (membrane-spanning component forming the proton channel)
The catalytic portion includes 5 different subunits (alpha, beta, gamma, delta, epsilon) assembled with stoichiometry of 3 alpha, 3 beta, and one each of the other three.
The proton channel consists of three main subunits (a, b, c).
This gene encodes the alpha subunit of the catalytic core.
Alternatively spliced transcript variants encoding the same protein have been identified.
Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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