Thermo Fisher Scientific COPE Monoclonal Antibody (9B6)
Thermo Fisher Scientific의 COPE Monoclonal Antibody (9B6)는 인간, 마우스, 랫트에 반응하는 단일클론 항체로 Western blot, IHC, ICC/IF, Flow cytometry에 적합합니다. E. coli 유래 COPE 단백질을 면역원으로 사용하며, 고순도 친화 크로마토그래피로 정제된 비결합형 항체입니다.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg / 1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9B6 |
| Immunogen | E. coli-derived human COPE recombinant protein (Position: E80–A308). Human COPE shares 89.5% amino acid sequence identity with mouse COPE. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2884066 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The COPE gene encodes the epsilon subunit of the coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and associates reversibly with Golgi non-clathrin-coated vesicles. It is essential for budding from Golgi membranes and for retrograde Golgi-to-ER transport of dilysine-tagged proteins. The complex includes alpha, beta, beta’, gamma, delta, epsilon, and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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