Thermo Fisher Scientific Ran Monoclonal Antibody (5D5)
Ran 단백질을 인식하는 Mouse IgG2b 단일클론 항체로 Western blot, ICC/IF, Flow cytometry에 사용 가능. 인간, 마우스, 랫트 반응성. 동결건조 형태로 제공되며 재구성 후 500 µg/mL 농도. RNA 및 단백질 핵수송 연구에 적합.
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Thermo Fisher Scientific Ran Monoclonal Antibody (5D5)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D5 |
| Immunogen | E. coli-derived human Ran recombinant protein (Position: A2–L216). Human Ran shares 100% amino acid sequence identity with both mouse and rat Ran. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | 0.05 mg sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2884070 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Ran (ras-related nuclear protein) is a small GTP-binding protein belonging to the RAS superfamily. It is essential for the translocation of RNA and proteins through the nuclear pore complex and is involved in the control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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